Detection method: quantitative real-time PCR
The kit is used to detect and quantify the BRAF V600E, E2, D, D2, K, R and M somatic mutations in human genomic DNA. The principle of detection is based on real-time PCR methodology with the use of fluorescently labelled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.
Single nucleotide mutation of the BRAF V600 gene leads to increased activation of the MAP kinase cascade, resulting in proliferation and dedifferentiation. However, the BRAF gene mutation alone is not sufficient to induce a malignant transformation. Its role lies more in the progression of the disease than in the initiation.
The occurrence of the BRAF V600E mutation was observed, for example, in melanoma, Langerhans’ ischemia of the pancreas, or ovarian and colon cancer. BRAF gene mutations are also associated with several tumors (malignant melanoma, non-Hodgkin’s lymphoma, thyroid papillary carcinoma, non-small cell lung carcinoma and lung adenocarcinoma). Before initiating treatment with vemurafenib or dabrafenib, patients should be confirmed with the validated test of the V600 mutation of the BRAF gene. Vemurafenib is not suitable for patients with malignant melanoma and a wild type variant of the BRAF gene.
Detected mutations in gene BRAF:
| Change of AMK | Change of nucleotide | Cosmic ID |
| p.V600E | c.1799T>A | 476 |
| p.V600E | c.1799_1800 TG>AA | 475 |
| p.V600D | c.1799_1800 TG>AT | 477 |
| p.V600D | c.1799_1800 TG>AC | 308550 |
| p.V600K | c.1798_1799 GT>AA | 473 |
| p.V600R | c.1798_1799 GT>AG | 474 |
| p.V600M | c.1798G>A | 1130 |
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3281-024 | gb ONCO BRAF (V600) | 24 |
Add to Quote Add to Quote |
| 3281-048 | gb ONCO BRAF (V600) | 48 |
Add to Quote Add to Quote |
1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
Shiping on dry ice from
€ 110 (EU)
For other destinations see
indicative list.
General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Your request has been sent.
Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
This product has not been validated for your cycler?
Send us the type of your cycler and we will find a solution for you.
Your request has been sent.
Thank you for your interest in our testing services. We will reply to your e-mail address as soon as possible.
Thank you for your inquiry
Thank you for your inquiry
Your registration has been sent.
Thank you for your inquiry
Price will be calculated based on your exact location within 2 working days.
ENG 
CZE 