The kit is used to detect and quantify the BRAF V600E, E2, D, D2, K, R and M somatic mutations in human genomic DNA. The principle of detection is based on real-time PCR methodology with the use of fluorescently labelled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.
Single nucleotide mutation of the BRAF V600 gene leads to increased activation of the MAP kinase cascade, resulting in proliferation and dedifferentiation. However, the BRAF gene mutation alone is not sufficient to induce a malignant transformation. Its role lies more in the progression of the disease than in the initiation.
The occurrence of the BRAF V600E mutation was observed, for example, in melanoma, Langerhans’ ischemia of the pancreas, or ovarian and colon cancer. BRAF gene mutations are also associated with several tumors (malignant melanoma, non-Hodgkin’s lymphoma, thyroid papillary carcinoma, non-small cell lung carcinoma and lung adenocarcinoma). Before initiating treatment with vemurafenib or dabrafenib, patients should be confirmed with the validated test of the V600 mutation of the BRAF gene. Vemurafenib is not suitable for patients with malignant melanoma and a wild type variant of the BRAF gene.
Detected mutations in gene BRAF:
| Change of AMK | Change of nucleotide | Cosmic ID |
| p.V600E | c.1799T>A | 476 |
| p.V600E | c.1799_1800 TG>AA | 475 |
| p.V600D | c.1799_1800 TG>AT | 477 |
| p.V600D | c.1799_1800 TG>AC | 308550 |
| p.V600K | c.1798_1799 GT>AA | 473 |
| p.V600R | c.1798_1799 GT>AG | 474 |
| p.V600M | c.1798G>A | 1130 |
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3281-024 | gb ONCO BRAF (V600) | 24 |
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| 3281-048 | gb ONCO BRAF (V600) | 48 |
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1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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