Detection method: reverse transcription + real-time PCR
This in vitro diagnostic kit is intended for prognosis assessment in CLL patients by analysis of cDNA (reverse transcribed RNA) samples of human B lymphocytes obtained from peripheral blood. The kit is based on reverse transcription together with quantitative real-time polymerase chain reaction (qRT-PCR) using fluorescently labelled probes.
Chronic lymphocytic leukemia (CLL) occurs mostly in elderly people (median age 65 years) and is the most common leukemia in Europe and North America where CLL comprises of about 30 % of leukemic diseases of adults. The frequency of CLL is approximately two-times higher in men than in women. IGHV mutational status analysis helps to better predict disease progression and patient survival.
Somatic hypermutation of IGHV is the physiological process that leads to an increase in antibody diversity. In patients with mutated IGHV, better prognosis with a slower progression of disease and a longer patient´s survival can be observed. In those with unmutated IGHV a worse clinical course and prognosis can be expected.The kit contains all components necessary for analysis of gene expression. Two of six genes are housekeeping genes (B2M, HPRT1) and three of them are target genes (LPL, ZAP70 and COBLL1). The remaining gene CD3D serves as contamination marker (sample contamination by T lymphocytes).
| Cat. No. | Product | No. of tests | Price |
|---|
| 3240-010 | gb ONCO CLL | 10 |
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1 CE IVD real-time PCR kit contains reagents to provide 30 PCR reactions (20 μl volume of each reaction) – test for 10 patients.
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General Terms and Conditions
Validated for:
ABI 7300 (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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