gb ONCO EGFR (T790M) kit enables the detection and quantification of the EGFR T790M (2369C>T, rs121434569) somatic mutation in the human genomic DNA. The detection principle is based on real-time PCR methodology with the use of fluorescently labeled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.
The Epidermal Growth Factor Receptor (EGFR) is expressed on the cell surface and has tyrosine kinase activity. EGFR regulates the cell cycle via the MAPK/ERK and PI3K/AKT/mTOR pathways. Activating mutations of EGFR leads to induction of its kinase activity, which results to uncontrolled cell proliferation.
This is used in the cancer treatment by so-called tyrosine kinase inhibitors (TKIs), which bind to the ATP receptor binding site and inhibit EGFR activity. On the other side, the T790M mutation found in exon 20 leads to the TKI resistance.
Anti-EGFR TKIs are particularly useful in the treatment of lung tumors, especially non-small cell lung cancer. However, resistance to TKI develops very quickly (median 9–13 months). Therefore, detection of the T790M mutation is required before the treatment with TKI-group medicinal products.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3245-024 | gb ONCO EGFR (T790M) | 24 |
Add to Quote Add to Quote |
| 3245-048 | gb ONCO EGFR (T790M) | 48 |
Add to Quote Add to Quote |
1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
Shiping on dry ice from
€ 110 (EU)
For other destinations see
indicative list.
General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
This product was developed within the project TH03010251 IVD PROBES that was co-funded by Technology Agency of the Czech Republic - www.tacr.cz (EPSILON Programme). 
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional
The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. BD analysis is generally conducted at the molecular level using a methodology that provides high sensitivity.
Your request has been sent.
Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
This product has not been validated for your cycler?
Send us the type of your cycler and we will find a solution for you.
Your request has been sent.
Thank you for your interest in our testing services. We will reply to your e-mail address as soon as possible.
Thank you for your inquiry
Thank you for your inquiry
Thank you for your inquiry
Your registration has been sent.
Thank you for your inquiry
Price will be calculated based on your exact location within 2 working days.
ENG 
CZE 