Detection method: quantitative real-time PCR
gb ONCO EGFR (T790M) kit enables the detection and quantification of the EGFR T790M (2369C>T, rs121434569) somatic mutation in the human genomic DNA. The detection principle is based on real-time PCR methodology with the use of fluorescently labeled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.
The Epidermal Growth Factor Receptor (EGFR) is expressed on the cell surface and has tyrosine kinase activity. EGFR regulates the cell cycle via the MAPK/ERK and PI3K/AKT/mTOR pathways. Activating mutations of EGFR leads to induction of its kinase activity, which results to uncontrolled cell proliferation.
This is used in the cancer treatment by so-called tyrosine kinase inhibitors (TKIs), which bind to the ATP receptor binding site and inhibit EGFR activity. On the other side, the T790M mutation found in exon 20 leads to the TKI resistance.
Anti-EGFR TKIs are particularly useful in the treatment of lung tumors, especially non-small cell lung cancer. However, resistance to TKI develops very quickly (median 9–13 months). Therefore, detection of the T790M mutation is required before the treatment with TKI-group medicinal products.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3245-024 | gb ONCO EGFR (T790M) | 24 |
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| 3245-048 | gb ONCO EGFR (T790M) | 48 |
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1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
This product was developed within the project TH03010251 IVD PROBES that was co-funded by Technology Agency of the Czech Republic - www.tacr.cz (EPSILON Programme). 
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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