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gb ONCO EGFR T790M

gb ONCO EGFR (T790M) kit enables the detection and quantification of the EGFR T790M (2369C>T, rs121434569) somatic mutation in the human genomic DNA. The detection principle is based on real-time PCR methodology with the use of fluorescently labeled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.

Clinical implications

The Epidermal Growth Factor Receptor (EGFR) is expressed on the cell surface and has tyrosine kinase activity. EGFR regulates the cell cycle via the MAPK/ERK and PI3K/AKT/mTOR pathways. Activating mutations of EGFR leads to induction of its kinase activity, which results to uncontrolled cell proliferation.

This is used in the cancer treatment by so-called tyrosine kinase inhibitors (TKIs), which bind to the ATP receptor binding site and inhibit EGFR activity. On the other side, the T790M mutation found in exon 20 leads to the TKI resistance.

Anti-EGFR TKIs are particularly useful in the treatment of lung tumors, especially non-small cell lung cancer. However, resistance to TKI develops very quickly (median 9–13 months). Therefore, detection of the T790M mutation is required before the treatment with TKI-group medicinal products.

Parametres of the diagnostic kit

qPCR assay + Master Mix
LOD 0.05% mutated EGFR on WT background at 100,000 copies in reaction
sample concentration 10-80 ng/µl
positive and negative controls are included
FAM channel detection
identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits

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Cat. No.	Product	No. of reactions	Price

3245-024

gb ONCO EGFR (T790M)

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3245-048

gb ONCO EGFR (T790M)

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1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

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About product

General Terms and Conditions

Validated for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

Select further cyclers

This product was developed within the project TH03010251 IVD PROBES that was co-funded by Technology Agency of the Czech Republic - www.tacr.cz (EPSILON Programme).

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