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gb ONCO EGFR T790M gb ONCO EGFR T790M

gb ONCO EGFR T790M

gb ONCO EGFR (T790M) kit enables the detection and quantification of the EGFR T790M (2369C>T, rs121434569) somatic mutation in the human genomic DNA. The detection principle is based on real-time PCR methodology with the use of fluorescently labeled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.

Clinical implications

The Epidermal Growth Factor Receptor (EGFR) is expressed on the cell surface and has tyrosine kinase activity. EGFR regulates the cell cycle via the MAPK/ERK and PI3K/AKT/mTOR pathways. Activating mutations of EGFR leads to induction of its kinase activity, which results to uncontrolled cell proliferation.

This is used in the cancer treatment by so-called tyrosine kinase inhibitors (TKIs), which bind to the ATP receptor binding site and inhibit EGFR activity. On the other side, the T790M mutation found in exon 20 leads to the TKI resistance.

Anti-EGFR TKIs are particularly useful in the treatment of lung tumors, especially non-small cell lung cancer. However, resistance to TKI develops very quickly (median 9–13 months). Therefore, detection of the T790M mutation is required before the treatment with TKI-group medicinal products.

Parametres of the diagnostic kit

  • qPCR assay + Master Mix
  • LOD 0.05% mutated EGFR on WT background at 100,000 copies in reaction
  • sample concentration 10-80 ng/µl
  • positive and negative controls are included
  • FAM channel detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3245-024 gb ONCO EGFR (T790M) 24
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3245-048 gb ONCO EGFR (T790M) 48
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Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Validated for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

Select further cyclers

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