PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.
gb OneStep IPC RT-qPCR Kit
gb OneStep IPC RT-qPCR Kit contains hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and M-MuLV reverse transcriptase and internal positive control (IPC). The IPC contained in the Master Mix is used to check the correctness of the PCR, allowing the user to distinguish a sample that appears to be negative due to poorly passed PCR, from a truly negative sample.
Description of the product
gb OneStep RT-qPCR Kit is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e.onestep RT-qPCR. No additional instrumentation is required; the onestep RT-qPCR analysis can be performed directly on any real-time PCR cycler simply by adding a thermal step for reverse transcription prior to a PCR amplification.
The IPC signal is detected in the HEX / JOE / VIC fluorescence channel. gb OneStep IPC RT-qPCR Kit does not contain any passive reference dye, it can be added to the reaction mixture separately (view Passive Reference Dye, Cat. No. 3010 – on request).
Parameters of the product
- Kit consists of 2 vials (OneStep IPC Buffer and OneStep IPC Enzyme). After mixing the 2 vials together you will get a complete OneStep IPC Master Mix.
- gb OneStep IPC RT-qPCR Kit is 2x concentrated and is sufficient for 50 reactions of 20 µl.
|Cat. No.||Product||No. of reactions||Price|
|3018-IPC||gb OneStep IPC RT-qPCR Kit||50||
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1 pack contains reagents to provide 50 reverse transcriptions (20 μl volume of each reaction).
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€ 100 (EU)
For other destinations see indicative list.