PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.
gb OneStep RT-qPCR Kit
gb OneStep RT-qPCR Kit contains hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and M-MuLV reverse transcriptase. gb OneStep RT-qPCR Kit does not contain any passive reference dye, it can be added to the reaction mixture separately (view Passive Reference Dye, Cat. No. 3010 – on request).
Description of the product
gb OneStep RT-qPCR Kit is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required; the onestep RT-qPCR analysis can be performed directly on any real-time PCR cycler simply by adding a thermal step for reverse transcription prior to a PCR amplification.
Parameters of the product
- Kit consists of 2 vials (OneStep Buffer and OneStep Enzyme). After mixing the 2 vials together you will get a complete OneStep Master Mix.
- gb OneStep RT-qPCR Kit is 2x concentrated and is sufficient for 50 reactions of 20 µl.
|No. of reactions
|gb OneStep RT-qPCR Kit
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1 pack contains reagents to provide 50 reverse transcriptions (20 μl volume of each reaction).
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€ 110 (EU)
For other destinations see indicative list.