gb OneStep RT-qPCR Kit
gb OneStep RT-qPCR Kit contains hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and M-MuLV reverse transcriptase. gb OneStep RT-qPCR Kit does not contain any passive reference dye, it can be added to the reaction mixture separately (view Passive Reference Dye, Cat. No. 3010 – on request).
Description of the product
gb OneStep RT-qPCR Kit is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required; the onestep RT-qPCR analysis can be performed directly on any real-time PCR cycler simply by adding a thermal step for reverse transcription prior to a PCR amplification.
Parameters of the product
- Kit consists of 2 vials (OneStep Buffer and OneStep Enzyme). After mixing the 2 vials together you will get a complete OneStep Master Mix.
- gb OneStep RT-qPCR Kit is 2x concentrated and is sufficient for 50 reactions of 20 µl.