Detection method: allelic discrimination
The CE IVD kit is used for determination of genotype of four polymorphisms according to guidelines CPIC and DPWG: *2A (c1905+1G>A), *13 (c1679T>G), HapB3 (c1236G>A) and c2846A>T in human genomic DNA. The principle of detection is based on real-time PCR with fluorescently labelled probes (allelic discrimination).
Fluoropyrimidines (5-fluorouracil and its prodrugs capecitabine and tegafur) are the basic drugs used in chemotherapy of gastrointestinal cancer. Their use is limited by development of many toxic effects including life-threatening myelosuppression. Dihydropyrimidine dehydrogenase (DPD) catalyzes the initial step of pyrimidines catabolism.
It inactivates fluoropyrimidine chemotherapeutics and its activity influences the frequency of toxicity. It is not recommended to administer the fluoropyrimidines to the patients with DPD deficiency. When studying polymorfisms of the gene for DPD (DPYD), the relationship between presence of particular mutations and decreased activity of DPD has been demonstrated.
According to recommendations of pharmacogenetics consortia CPIC (from 2017) and DPWG (from 2019), the testing of presence of four polymorphisms in DPYD gene: *2A (c1905+1G>A), *13 (c1679T>G), HapB3 (c1236G>A) and c2846A>T is recommended before administration of the above substances. Insufficient evidence has been gathered for other polymorphisms in relation of their effect on DPD enzyme activity.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3255-025 | gb PHARM DPYD | 25 |
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1 CE IVD kit contains reagents to provide 25 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX Opus 96 (Bio-Rad)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Storage at -20 °C
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Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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