Detection method: allelic discrimination
This in vitro diagnostic kit is intended for detection of TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in TPMT gene in human genomic DNA by realtime PCR method (allelic discrimination). The kit allows the determination of haplotypes TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in homozygous or heterozygous form. The kit does not distinguish genotype *1/*3A (two mutations in cis form) from 3B*/*3C (the incidence of the composite heterozygote *3B/3C* is unlikely in Caucasians).
Thiopurine S-methyltransferase (TPMT) is an essential enzyme for biodegradation of thiopurines. Antitumor drugs thioguanine and mercaptopurine, related to thiopurines, are used especially in hematooncology. Another thiopurine, azathioprine, is an immunosuppressive agent intended for the treatment of autoimmune diseases and prevention of transplant rejection.
The thiopurine drug side effects include neurotoxicity, hepatotoxicity, myelosuppression, mucositis and others. The biotransformation activity of the TPMT enzyme may be reduced due to the presence of the single nucleotide polymorphisms in the coding region of the TPMT gene. The most studied deficient alleles are TPMT*, TPMT*3A, TPMT*3B and TPMT*3C. Limited thiopurine biotransformation leads to the escalation of side effects of treatment. Upon detection of heterozygote it is recommended to reduce the dose of the drug to 30-70%, in the case of the homozygote and the composite heterozygote it is better to choose an alternative treatment.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3217-025 | gb PHARM TPMT | 25 |
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| 3217-050 | gb PHARM TPMT | 50 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
ABI 7500/7500 Fast (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
gb Elite PCR Master Mix gb Elite PCR Master Mix falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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