gb SG PCR Master Mix

gb SG PCR Master Mix

gb SG PCR Master Mix is intended for real-time PCR analysis using sybrgreen instead of fluorescently labelled probe. gb SG PCR Master Mix can also be used for the end-point PCR analysis. Beneficial feature is enhanced resistance to PCR inhibitors. It is not intended for use in diagnostics.

Description of the product

gb SG PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Parameters of the product

  • gb SG PCR Master Mix is a 2× concentrated solution.
  • It contains all the components necessary for PCR performance.
  • Due to its special composition there is an increased resistance to PCR inhibitors.
  • It is suitable for PCR performance detection with the help of fluorescent dye in the FAM channel.
  • Polymerase is a hot-start type with a short activation time (3 min / 95 °C), with 5´-3´ polymerase and exonuclease activity, 3´-5´ exonuclease activity is not present.
  • Individual features of Master Mixes from our offer for your comparison can be found on our webpages.


gb PCR Master Mix by application
gb SG
end-point PCR, common PCR amplification
real-time PCR without probes

real-time PCR with hydrolysis probes
real-time PCR with LNA probes
real-time PCR with hybridization probes
real-time PCR with High Resolution Melting Analysis
real-time PCR with low DNA samples
real-time PCR with inhibited samples

Cat. No. Product No. of reactions Price
3005 gb SG PCR Master Mix 100
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

General Terms and Conditions

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Real-Time PCR Troubleshooting

Real-Time PCR Troubleshooting

PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.

gb HRM PCR Master Mix

gb HRM PCR Master Mix gb HRM PCR Master Mix

gb HRM PCR Master Mix falls into a group of Dye-Based Real-time PCR Master Mixes. The product is intended for amplification of DNA with subsequent High Resolution Melting (HRM) analysis. HRM analysis is an efficient melt curve analysis method for genotyping, mutation scanning prior to sequencing analysis, DNA mapping, species identification or DNA methylation analysis etc.