gb StarGreen PCR Mix
gb StarGreen PCR Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, SYBR Green and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
Purpose of the product
gb StarGreen PCR Mix is intended for real-time PCR analysis using SYBR Green instead of fluorescently labelled probe. gb StarGreen PCR Mix can also be used for the end-point PCR analysis. Beneficial feature is enhanced resistance to PCR inhibitors. The PCR Mix is intended for research use only (RUO).
Parameters of the product
- PCR Mix is a 2× concentrated.
- It contains all the components necessary for PCR performance.
- It is suitable for PCR detection using SYBR Green I.
- Due to its special composition there is an increased resistance to PCR inhibitors.
- Polymerase is a hot-start type with a short activation time (2 min / 95 °C), with 5´-3´ polymerase and exonuclease activity, 3´-5´ exonuclease activity is not present.
- The DNA polymerase exhibits high processivity.
gb PCR Mix by application
|
gb StarGreen PCR Mix |
| end-point PCR, common PCR amplification |
✓
|
| internal positive control included |
|
| real-time PCR without probes |
✓
|
| real-time PCR with hydrolysis probes |
|
| real-time PCR with low DNA samples |
✓
|
| real-time PCR with inhibited samples |
✓
|
| uracil-DNA glykosylase included |
|