ngs ONCO anti-EGFR ngs ONCO anti-EGFR

ngs ONCO anti-EGFR

Detection method: NGS

This kit is intended for target amplification of hot-spot areas in selected oncogenes relevant for target therapy of oncological patients (colorectal cancer, non-small cell lung cancer). It is designed to prepare a library of samples for sequencing on the Illumina MiSeq® platform. The kit includes free use of the very user-friendly ERMI evaluation software.

Clinical implications

Diagnostic kit ngs ONCO anti-EGFR is a CE-IVD certified test that consists of a clinically relevant gene panel for target-therapy optimization of oncologic patients. It helps to determine suitability of deployed monoclonal antibodies Cetuximab (Erbitux) and Panitumumab (Vectibix) in colorectal cancer (CRC) that target EGFR (Epidermal growth factor receptor) protein by confirming/excluding mutations in KRAS, NRAS, and BRAF genes, because patients with these mutations do not profit from anti-EGFR treatment.

Furthermore, kit ngs ONCO anti-EGFR determines the suitability of EGFR-inhibitors Erlotinib (Tarceva), Gefitinib (Iressa), Afatinib (Gilotrif), Osimertinib (Tagrisso), Dacomitinib (Vizimpro), and Osimertinib (Tagrisso), for patients with non-small cell lung cancer (NSCLC). The kit is also intended for supportive diagnostics for the use of targeted therapy in NSCLC and melanomas caused by mutations in the BRAF gene where treatment with the inhibitor Dabrafenib (Tafinlar), Vemurafenib (Zelboraf) or Encorafenib (Braftovi) is used.

Parameters of the diagnostic kit

  • Diagnostic kits for next-generation sequencing on Illumina MiSeq® platform
  • Up to 64 samples per sequencing run (when used with v2 300 cycles chemistry)
  • Panel of 8 relevant genes for molecular diagnostics of somatic mutations in colorectal cancer, lung cancer, and melanomas using the anti-EGFR antibody therapy
  • Carefully designed primers for single-tube amplicon multiplex PCR library preparation providing uniform coverage
  • Suitable with the FFPE samples and input DNA in amounts as low as 20 ng
  • Straightforward library preparation protocol with short hands-on time
  • Developed in collaboration with experts from the Clinic of Oncology, General University Hospital in Prague
  • Bioinformatic software solution provides user friendly data analysis and generates a report


Panel of genes included in ngs ONCO anti-EGFR  diagnostic kit:

KRAS chr 12 exon 2 exon 3 exon 4
BRAF chr 7 exon 11 exon 15
NRAS chr 1 exon 2 exon 3 exon 4
EGFR chr 7 exon 18 exon 19 exon 20 exon 21
PIK3CA chr 3 exon 21
AKT1 chr 14 exon 3
HER2/ERBB2 chr 17 exon 24
SMAD4 chr 18 exon 12
Cat. No. Product No. of reactions Price
3301-024 ngs ONCO anti-EGFR 24
Add to Quote
Add to Quote

Get this product FREE
Get up to 2 different free samples. Pay only shipping.

Order for free
3301-064 ngs ONCO anti-EGFR 64
Add to Quote
Add to Quote

Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Automatic evaluation : Yes

Validated for:

Illumina MiSeq