NGS primers can be ordered if they make a group of at least 24 pieces of oligonucleotides, up to 99 nucleotides long, provided that the shortest and the longest sequence in the group differs by 15 nucleotides maximum.
Shiping at ambient temperature from
€ 40 (EU)
For other destinations see
The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.