Nucleic Acid (DNA/RNA) Extraction

Nucleic acid extraction can be tedious and time-consuming. It is the most crucial method used in molecular biology and is the starting point for many downstream processes and product development including diagnostic kits. DNA and RNA can be isolated from any biological material such as living or conserved tissues, cells, virus particles, or other samples for analytical or preparative purposes.

Generally, successful nucleic acid purification requires four important steps:

effective disruption of cells or tissue
denaturation of nucleoprotein complexes
inactivation of nucleases, for example, RNase for RNA extraction and DNase for DNA extraction
removal of contamination such as proteins, carbohydrates, lipids, or other nucleic acids (for example, DNA free of RNA or RNA free of DNA)

Generi Biotech provides nucleic acid extraction as part of complete projects and as a standalone service. Simply provide us with your sample and we will do the rest — delivering you a high quality extracted product or information about the extracted material necessary for downstream applications. Regulatory grade extraction services (GLP-compliant) are also available.

Free up your bench time and let Generi Biotech perform your DNA & RNA extractions!

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gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.