Categories

Nucleic Acid (DNA/RNA) Extraction

Nucleic acid extraction can be tedious and time-consuming. It is the most crucial method used in molecular biology and is the starting point for many downstream processes and product development including diagnostic kits. DNA and RNA can be isolated from any biological material such as living or conserved tissues, cells, virus particles, or other samples for analytical or preparative purposes.

Generally, successful nucleic acid purification requires four important steps:

  1. effective disruption of cells or tissue
  2. denaturation of nucleoprotein complexes
  3. inactivation of nucleases, for example, RNase for RNA extraction and DNase for DNA extraction
  4. removal of contamination such as proteins, carbohydrates, lipids, or other nucleic acids (for example, DNA free of RNA or RNA free of DNA)

Generi Biotech provides nucleic acid extraction as part of complete projects and as a standalone service. Simply provide us with your sample and we will do the rest — delivering you a high quality extracted product or information about the extracted material necessary for downstream applications.  Regulatory grade extraction services (GLP-compliant) are also available.

Free up your bench time and let Generi Biotech perform your DNA & RNA extractions!

Useful links
Recommended products
gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More