Passive reference dye
Passive reference dye is an inert dye which serves as inner reference dye for fluorescence signal normalization between the wells which can occur as a result of errors in pipetting or under the influence of device limitations.
Passive reference dye is used especially in real-time PCR devices from Applied Biosystems and Stratagene.
Product specifications
- It is used for fluorescence signal normalization to significantly increase the accuracy of measured data
- Excitation wavelength of the passive reference dye is 575 nm and emission wavelength is 605 nm