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Phosphate

Modification by group: Phosphorylation

Position of modification: 3´, 5´

It can be linked to an oligonucleotide at the 5 ‘end and the 3’ end. The 5 ‘end is used for ligation. The 3 ‘end serves as a 3’ polymerase extension blocker.

Cat. No.	Modification	Scale	Price

1110-040

5' Phosphate

40 nmol

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1110-200

5' Phosphate

200 nmol

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1110-001

5' Phosphate

1 micromol

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Oligo price = price of sequence without modification + surcharge for modification

Shiping at ambient temperature from € 45 (EU)
For other destinations see indicative list.

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gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.