Plasmid amplification is provided in Escherichia coli bacteria cells. Plasmid linearization by restriction cleavage can be ordered as a follow-up service. Such an operation is recommended especially when the plasmid is used as a PCR standard. Amplified plasmids are delivered either in midiprep or maxiprep quantities. Typical yields are stated below.
Plasmid isolation yield depends on many factors: on the sequence coding for DNA replication origin (ORI sequence), on the size of the plasmid, and on the type of cloned DNA fragment (e.g. inserted fragment of shRNA/miRNA can significantly lower the yield). To increase final yield, it is recommended to provide us with the information whether the plasmid is considered either as “high copy” or “low/very low copy”, according to the reference table.
If the plasmid is inappropriately categorized, we do not take responsibility concerning the total yield. If the customer provides us with detailed information on the plasmid (length, name of the original vector and map of the plasmid) we will categorize it correctly by ourselves.
Plasmid can be sent either in the form of a solution or as a dried drop on a filter paper.
Customers should specify the gene for antibiotics resistance and categorize the plasmid into either “high copy” or “low/very low copy” group.
Finalized plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request). We provide you with information about plasmid concentration and purity.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1851-001 | Midiprep “high copy” |
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| 1852-001 | Midiprep "low/very low copy" |
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| 1853-001 | Maxiprep "high copy" |
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| 1854-001 | Maxiprep "low/very low copy" |
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Shiping on dry ice from
€ 110 (EU)
For other destinations see
indicative list.
General Terms and Conditions
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Within 15 business days upon receiving order
Shipping
Plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request)
Recommended storage
-20° C (in a refrigerator)
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