Plasmid isolation yield depends on many factors: on the sequence coding for DNA replication origin (ORI sequence), on the size of the plasmid, and on the type of cloned DNA fragment (e.g. inserted fragment of shRNA/miRNA can significantly lower the yield). To increase final yield, it is recommended to provide us with the information whether the plasmid is considered either as “high copy” or “low/very low copy”, according to the reference table.
If the plasmid is inappropriately categorized, we do not take responsibility concerning the total yield. If the customer provides us with detailed information on the plasmid (length, name of the original vector and map of the plasmid) we will categorize it correctly by ourselves.
Requirements for the material
Plasmid can be sent either in the form of a solution or as a dried drop on a filter paper.
- Minimal amount of plasmid in solution: 100 ng
- Minimal amount of plasmid on filter paper: 1 µg
- Minimal purity (A260/A280) at least 1.8
Customers should specify the gene for antibiotics resistance and categorize the plasmid into either “high copy” or “low/very low copy” group.
Finalized plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request). We provide you with information about plasmid concentration and purity.