gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.
This service follows plasmid amplification and isolation. Linear DNA provides more reproducible and accurate results. Linearization is performed using restriction endonucleases. The cleaved product is further purified and quantified.
- isolate of a linearized plasmid in the minimal amount of 10 μg
- concentration min 1E10 copies/μl
- volume of 200 μl
The cleaved product is further purified and quantified.
Finalized plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request). We provide you with information about plasmid concentration and purity.
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