If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.
This service follows plasmid amplification and isolation. Linear DNA provides more reproducible and accurate results. Linearization is performed using restriction endonucleases. The cleaved product is further purified and quantified.
The cleaved product is further purified and quantified.
Finalized plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request). We provide you with information about plasmid concentration and purity.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1850 | Plasmid linearization |
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General Terms and Conditions
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional
The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. BD analysis is generally conducted at the molecular level using a methodology that provides high sensitivity.
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Dispatch date
Within 20 business days upon receiving order
Shipping
Plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request)
Recommended storage
-20° C (in a refrigerator)
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