HEX-BBQ650 is a real-time PCR dual-labeled probe with HEX fluorophore and BBQ650 dark quencher. It can be used as an alternative to BHQ2 quencher due to its broader absorption spectrum and higher stability. The probe is most commonly used as a Taqman-type hydrolysis probe providing highly sensitive and specific detection of target DNA. It is suitable for multiplex analyses with low background and high signal intensity and is compatible with a wide range of qPCR instruments.
Cy3 λex = 537 nm λem = 556
BHQ2 absorption maximum = 650 nm
| Cat. No. | Product | Amount | Price |
|---|
| 1533-005 | Probe HEX-BBQ650 | 5 nmol |
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| 1533-010 | Probe HEX-BBQ650 | 10 nmol |
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| 1533-020 | Probe HEX-BBQ650 | 20 nmol |
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| 1533-050 | Probe HEX-BBQ650 | 50 nmol |
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Quencher BBQ is covered by US Patent 7,879,986.
Shiping at ambient temperature from
€ 45 (EU)
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indicative list.
General Terms and Conditions
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Term A: quick term not available
Term B: products are dispatched within the next 5 full business days after receiving the order
Shipping
At room temperature
Storage recommendation
Dried oligonucleotides: at 4 °C (in a refrigerator)
Dissolved: -20°C (in a freezer), we recommend to store in aliquotes
In a dark place (without exposure to the light)
Product expiry date
24 months from the date of manufacture (provided the product is stored as recommended)
The provided information is only indicative and is always subject to the specific content and extent of an order.
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