STANDARD MMR BCR-ABL MAJOR represents RNA with the precisely defined copy number ratio of BCR-ABL1 MAJOR (b3a2) and reference genes ABL1 and GUSB transcripts. Major molecular response (MMR) corresponds to BCR-ABL1 0.1% International Scale (IS) transcript levels. STANDARD MMR BCR-ABL MAJOR is used to convert BCR-ABL1 values to IS.

Clinical implication

The BCR-ABL1 MAJOR fusion gene variant occurs in 95% patients with chronic myelogenous leukemia (CML) and in 25% patients with acute lymphocytic leukemia (ALL). The chromosomal aberration leads to the dysregulation of the tyrosine kinase signaling pathway and to the manifestation of the oncological disease.

The response of the patient to the treatment and the minimal residual disease is monitored under recommendation through the quantification of the b2a2 (e13a2) and b3a2 (e14a2) BCR-ABL1 MAJOR fusion transcripts related to the reference gene transcripts. The conversion of the results into the International Scale (IS) of treatment response is enabled by using STANDARD MMR BCR-ABL MAJOR, which is verified for such purpose by the National Reference Laboratory of the Institute of Hematology and Blood Transfusion, Prague.

Product parameters

  • kit contents – 4 x 20 µl of STANDARD MMR BCR-ABL MAJOR with LOT specific verified copy number ratio of BCR-ABL1 to ABL1 and GUSB
  • 5 µl input into one RT-qPCR reaction per run
  • verified for conversion of results to IS
  • recommended use with kits gb ONCO BCR-ABL MAJOR/ABL cat. no.  3243-048, 3243-096) and gb ONCO BCR-ABL MAJOR/GUSB (cat. no. 3249-048, 3249-096)
Cat. No. Product Amount Price
3280-004 STANDARD MMR BCR-ABL MAJ 4×20 µl
Add to Quote
Add to Quote

Shiping on dry ice from € 105 (EU)
For other destinations see indicative list.

Validated for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

Select further cyclers

Useful links
Recommended products
Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional