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Viral shedding

Oncolytic viruses, which include both naturally occurring wild-type viruses/attenuated viruses and genetically modified viruses, have recently been developed for use in innovative cancer therapies. Genetically modified oncolytic viruses possess the unique ability to replicate conditionally as a unique gene therapy product. Since oncolytic viruses exhibit prolonged persistence in patients, viral shedding and transmission to third parties should be major concerns for clinical trials along with the clinical safety and efficacy.

Shedding is linked to biodistribution studies but unlike biodistribution, which describes how a product is spread within the animal model’s or patient’s body from the site of administration, shedding describes how it is excreted or released from the body.

In Generi Biotech, we have vast experience with qPCR testing in different matrices, most commonly in:

  • excreta (feces)
  • secreta (urine, saliva, nasopharyngeal fluids etc.)
  • skin-related tissue biopsies (pustules, sores, wounds)
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gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

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Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

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