Available synthesis scales
20 nmol, 40 nmol, 200 nmol, 1 μmol a 10 μmol
The standard length range of synthesized oligo is 2-99 bases. It is possible to synthesize strings with a length of 100 and more but must be consulted.
For unmodified oligonucleotides in 20 nmol scale, synthesis is available up to 50 bases in length.
A – preferred synthesis
B – common synthesis more about delivery time
Oligonucleotides may contain degeneration in any position. Degeneration can be performed as a mixture of bases or insertion of inosine into the chain. Insertion of inosine into a string: see the modification Inosin.
Marking a degenerate position in the order:
|I = inosin
||R = A, G
|N = A,C,G,T
||Y = C,T
|V = G,A,C
||M = A,C
|D = G,A,T
||K = G,T
|H = A,T,C
||S = G,C
|B = G,T,C
||W = A,T
The final product
The quantity of oligonucleotides is determined by UV spectrophotometry and expressed in nanomoles, optical units and micrograms. We standardly supply oligonucleotides in the dried state. Some oligonucleotides are delivered in an unexpected state for technological reasons.
A standard part of the production process is standard purification (desalination, removal of chemical impurities). Oligonucleotides of standard lengths (up to 40 bases) are of such quality that it is not necessary to purify for conventional molecular biological applications.
When ordering multiple chains of scale 40 nmol a discount is applied in the following discount bands:
a) OLIGO 10+ (10 – 49 pcs oligo)
b) OLIGO 50+ (50 – 149 pcs oligo)
c) OLIGO 150+ (150 pcs oligo and more)
To calculate the price of a specific order, use the oligocalculator (after login) or send a request.