DNA/RNA integrity

DNA/RNA integrity

Integrity of DNA/RNA is very important test needed according to the Europe pharmacopeia chapter 5.14.

Mostly the test can be done as agarose gel electrophoresis or using advanced techniques such as PCR or fragment analysis.

All the tests are according to the Europe pharmacopeia test 2.6.21.

To this test belong also plasmid DNA conformation test, because  Stability of plasmid DNA depends on its conformation and the right conformation  is required (e.g. supercoiled form).

For RNA integrity determination of RIN is important as well.

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Dual-labeled hydrolysis probes

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The technology of dual-labeled hydrolysis probes (also called TaqMan® probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave dual-labeled hydrolysis probes. Hydrolysis probes are labeled at the 5´ end with a fluorescent dye (reporter) and at the 3´ end with a quencher.

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