Modification by group: Fluorophores

Position of modification:

May be attached to the 5′ end of the oligonucleotide.

Absorption maximum: 547 nm

Emission maximum: 574 nm

Cat. No. Modifikace Scale Price
1124-5 5' Dy547 40 nmol
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1124-5 5' Dy547 200 nmol
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Modification is limited and can be performed upon consultation; precise price quotation can be prepared after providing particular sequences.

Oligo price = price of sequence without modification + surcharge for modification

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The technology of dual-labeled hydrolysis probes (also called TaqMan® probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave dual-labeled hydrolysis probes. Hydrolysis probes are labeled at the 5´ end with a fluorescent dye (reporter) and at the 3´ end with a quencher.

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