gb Easy PCR Master Mix

gb Easy PCR Master Mix is intended for research PCR and real-time PCR applications with fluorescently labelled probes. Master Mix is suitable e.g. for gene expression quantification or SNP detection. It is not intended for use in diagnostics.

Description of the product

gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Parameters of the product

  • Master Mix is a 2× concentrated solution.
  • It contains all the components necessary for PCR performance.
  • It is specified especially for real-time PCR applications demanding high sensitivity, for example microbial DNA detection and somatic mutations detection. Master Mix is enriched with components which preventPCR reaction inhibition through which higher reliability of the detection is ensured.
  • Polymerase is a hot-start type with a short activation time (3 min / 95 °C), with 5´-3´ polymerase and exonuclease activity, 3´-5´ exonuclease activity is not present.

 

gb PCR Master Mix by application
gb Easy
end-point PCR, common PCR amplification
real-time PCR without probes
real-time PCR with hydrolysis probes

real-time PCR with LNA probes

real-time PCR with hybridization probes
real-time PCR with High Resolution Melting Analysis
real-time PCR with low DNA samples
real-time PCR with inhibited sampels
Cat. No. Product No. of reactions Price
3006 gb Easy PCR Master Mix 100
Add to Quote

1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).

Useful links
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