gb GENETIC human DNA

Detection method: real-time PCR

The kit is intended for detection of the specific section of the POLR2A gene in the human genomic DNA. Detection is based on quantitative real-time polymerase chain reaction (qPCR) using fluorescently labelled probes.

Clinical implications

The quantification of human DNA is significant especially in the field of forensic analysis but it could be also exploitable in any other discipline dealing with human DNA analysis. It is essential before sample analysis, when it is important to find out if human DNA is present in the sample and possibly in which amount. The POLR2A gene encodes for the largest subunit of RNA polymerase II, an enzyme responsible for synthesizing messenger RNA in eukaryotes.

Parameters of the diagnostic kit

  • RUO
  • ready-to-use assay
  • positive and negative controls included
  • detection in FAM channel
  • internal positive control in HEX channel
Cat. No. Product No. of reactions Price
3242 gb GENETIC human DNA 100
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1 kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).

Validated for:

CFX96/96Touch (Bio-Rad)

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The technology of dual-labeled hydrolysis probes (also called TaqMan® probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave dual-labeled hydrolysis probes. Hydrolysis probes are labeled at the 5´ end with a fluorescent dye (reporter) and at the 3´ end with a quencher.

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