Categories

gb GENETIC Human DNA gb GENETIC Human DNA

gb GENETIC Human DNA

Detection method: real-time PCR

This kit is intended for detection of the specific section of the POLR2A gene in the human genomic DNA. Detection is based on quantitative real-time polymerase chain reaction (qPCR) using fluorescently labelled probes.

Clinical implications of the RUO kit

The quantification of human DNA is significant especially in the field of forensic analysis but it could be also exploitable in any other discipline dealing with human DNA analysis. It is essential before sample analysis, when it is important to find out if human DNA is present in the sample and possibly in which amount. The POLR2A gene encodes for the largest subunit of RNA polymerase II, an enzyme responsible for synthesizing messenger RNA in eukaryotes.

Parameters of the real-time PCR diagnostic kit

  • ready-to-use assay
  • positive and negative controls included
  • detection in FAM channel
  • internal positive control in HEX channel
Cat. No. Product No. of reactions Price
3142 gb GENETIC Human DNA 100
Add to Quote
Add to Quote

1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

    General Terms and Conditions

    Verified for:

    CFX96/96Touch (Bio-Rad)

    Select further cyclers

    Useful links
    Recommended products
    Dual-labeled hydrolysis probes

    Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

    The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

    More
    Recommended articles