gb HEMO FII (G20210A)

gb HEMO FII (G20210A)

Detection method: allelic discrimination, real-time PCR

The kit is intended for detection of mutation G20210A in coagulation factor II in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications

Coagulation factor II (prothrombin) is a glycoprotein involved in the process of blood coagulation. Prothrombin is converted to thrombin, which cleaves fibrinogen to fibrin monomers (necessary for blood coagulation). G20210A mutation is located in the prothrombin gene promotor region and causes prothrombin overactivation which leads to increased risk of blood clot creation.

Parameters of the diagnostic kit

  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3201-025 gb HEMO FII (G20210A) 25
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3201 gb HEMO FII (G20210A) 100
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1 kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Validated for:

ABI 7500/7500 Fast (ABI)

ABI 7900HT (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

MIC (BMS)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

SmartCycler (Cepheid)

Stratagene Mx3000P/Mx30005P (Agilent Technologies)

Select further cyclers

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