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gb HEMO FII (G20210A)

Detection method: allelic discrimination, real-time PCR

This in vitro diagnostic kit is intended for detection of mutation G20210A in coagulation factor II in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications of the CE IVD kit

Coagulation factor II (prothrombin) is a glycoprotein involved in the process of blood coagulation. Prothrombin is converted to thrombin, which cleaves fibrinogen to fibrin monomers (necessary for blood coagulation). G20210A mutation is located in the prothrombin gene promotor region and causes prothrombin overactivation which leads to increased risk of blood clot creation.

Parameters of the real-time PCR diagnostic kit

ready-to-use assay
sample concentration 1-100 ng/µl
positive and negative controls included
FAM and HEX channels detection
identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits

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Cat. No.	Product	No. of reactions	Price

3201-025	gb HEMO FII (G20210A)	25	Add to Quote Add to Quote
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3201

gb HEMO FII (G20210A)

100

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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

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About product

General Terms and Conditions

Validated for:

ABI 7500/7500 Fast (ABI)

ABI 7900HT (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

MIC (BMS)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

SmartCycler (Cepheid)

Stratagene Mx3000P/Mx30005P (Agilent Technologies)

Select further cyclers

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.