Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of mutation A1298C in MTHFR gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Methylenetetrahydrofolate reductase (MTHFR) is the main egulatory enzyme involved in homocysteine and folic acid (folates) metabolisms. This mutation leads to enzyme activity reduction, increase of homocysteine level in blood and methionine deficiency. However, lower catalytic activity of MTHFR enzyme means higher utilisation of 5, 10-methylenetetrahydrofolate in DNA synthesis or repair.
The risk of venous thrombosis increases in combination with other risk factors (e.g. mutation in MTHFR gene – C677T, mutation G20210A in factor II, Leiden mutation, long-time immobility, incorrect regimen, hormonal contraception) and grows with the number of mutated alleles in genotype.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3203-025 | gb HEMO MTHFR (A1298C) | 25 |
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| 3203 | gb HEMO MTHFR (A1298C) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
ABI 7500/7500 Fast (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
Stratagene Mx3000P/Mx30005P (Agilent Technologies)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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