
Dual-labeled hydrolysis probes
The technology of dual-labeled hydrolysis probes (also called TaqMan® probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave dual-labeled hydrolysis probes. Hydrolysis probes are labeled at the 5´ end with a fluorescent dye (reporter) and at the 3´ end with a quencher.