gb ONCO BRAF (V600E)

gb ONCO BRAF (V600E)

Detection method: quantitative real-time PCR

The kit is used to detect and quantify the BRAF V600E, E2, D somatic mutation in human genomic DNA. The detection principle is based on real-time polymerase chain reaction (qPCR) using fluorescently labeled probes and allele specific primers.

Clinical implications

Single nucleotide mutation of the BRAF V600E gene leads to increased activation of the MAP kinase cascade, resulting in proliferation and dedifferentiation. However, the BRAF gene mutation alone is not sufficient to induce a malignant transformation, its role lies more in the progression of the disease than in the initiation.

The occurrence of the BRAF V600E mutation was observed, for example, in melanoma, Langerhans’ ischemia of the pancreas, or ovarian and colon cancers. BRAF gene mutations are also associated with a number of tumors (malignant melanoma, non-Hodgkin’s lymphoma, thyroid papillary carcinoma, non-small cell lung carcinoma and lung adenocarcinoma). Prior to initiating treatment with vemurafenib or dabrafenib, patients should be confirmed with the validated test of the V600 mutation of the BRAF gene. Vemurafenib is not suitable for patients with malignant melanoma and a wild type variant of the BRAF gene.

Parameters of the diagnostic kit

  • ready-to-use assay
  • LOD 0.05% mutated BRAF on WT background at 100,000 copies in reaction
  • sample concentration 4-80 ng/µl
  • positive and negative controls included
  • FAM channel detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3241-025 gb ONCO BRAF (V600E) 25
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3241-050 gb ONCO BRAF (V600E) 50
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1 kit contains reagents to provide 25 or 50 PCR reactions (25 μl volume of each reaction).

Validated for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

Select further cyclers

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