Detection method: quantitative real-time PCR
This in vitro diagnostic kit is used to detect and quantify the BRAF V600E, E2, D and D2 somatic mutations in human genomic DNA. The detection principle is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes and allele specific primers.
Single nucleotide mutation of the BRAF V600E gene leads to increased activation of the MAP kinase cascade, resulting in proliferation and dedifferentiation. However, the BRAF gene mutation alone is not sufficient to induce a malignant transformation, its role lies more in the progression of the disease than in the initiation.
The occurrence of the BRAF V600E mutation was observed, for example, in melanoma, Langerhans’ ischemia of the pancreas, or ovarian and colon cancers. BRAF gene mutations are also associated with a number of tumors (malignant melanoma, non-Hodgkin’s lymphoma, thyroid papillary carcinoma, non-small cell lung carcinoma and lung adenocarcinoma). Prior to initiating treatment with vemurafenib or dabrafenib, patients should be confirmed with the validated test of the V600 mutation of the BRAF gene. Vemurafenib is not suitable for patients with malignant melanoma and a wild type variant of the BRAF gene.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3241-024 | gb ONCO BRAF (V600E) | 24 |
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| 3241-048 | gb ONCO BRAF (V600E) | 48 |
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1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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