gb OneStep RT-qPCR Kit

gb OneStep RT-qPCR Kit contains hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and M-MuLV reverse transcriptase. gb OneStep RT-qPCR Kit does not contain any passive reference dye, it can be added to the reaction mixture separately see Passive Reference Dye, Cat. No. 3010 – on request).

Description of the product

gb OneStep RT-qPCR Kit is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e.onestep RT-qPCR. No additional instrumentation is required; the onestep RT-qPCR analysis can be performed directly on any real-time PCR cycler simply by adding a thermal step for reverse transcription prior to a PCR amplification.

Parameters of the product

  • Kit consists of 2 vials (OneStep Buffer and OneStep Enzyme). After mixing the 2 vials together you will get a complete OneStep Master Mix.
  • gb OneStep RT-qPCR Kit is 2x concentrated and is sufficient for 50 reactions of 20 µl.
Cat. No. Product No. of reactions Price
3018 gb OneStep RT-qPCR Kit 50
Add to Quote

1 tube contains reagents to provide 50 reverse transcriptions (20 μl volume of each reaction).

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