Detection method: allelic discrimination

The kit is intended for detection of TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in TPMT gene in human genomic DNA by realtime PCR method (allelic discrimination). The kit allows the determination of haplotypes TPMT*2, TPMT*3A, TPMT*3B and TPMT*3C in homozygous or heterozygous form. The kit does not distinguish genotype *1/*3A (two mutations in cis form) from 3B*/*3C (the incidence of the composite heterozygote *3B/3C* is unlikely in Caucasians).

Clinical implications

Thiopurine S-methyltransferase (TPMT) is an essential enzyme for biodegradation of thiopurines. Antitumor drugs thioguanine and mercaptopurine, related to thiopurines, are used especially in hematooncology. Another thiopurine, azathioprine, is an immunosuppressive agent intended for the treatment of autoimmune diseases and prevention of transplant rejection.

The thiopurine drug side effects include neurotoxicity, hepatotoxicity, myelosuppression, mucositis and others. The biotransformation activity of the TPMT enzyme may be reduced due to the presence of the single nucleotide polymorphisms in the coding region of the TPMT gene. The most studied deficient alleles are TPMT*, TPMT*3A, TPMT*3B and TPMT*3C. Limited thiopurine biotransformation leads to the escalation of side effects of treatment. Upon detection of heterozygote it is recommended to reduce the dose of the drug to 30-70%, in the case of the homozygote and the composite heterozygote it is better to choose an alternative treatment.

Parameters of the diagnostic kit

  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3217-025 gb PHARM TPMT 25
Add to Quote
3217-050 gb PHARM TPMT 50
Add to Quote

1 kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).

Validated for:

ABI 7500/7500 Fast (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)


RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

Select further cyclers

Useful links
Recommended products
Recommended products
gb Easy PCR Master Mix

gb Easy PCR Master Mix gb Easy PCR Master Mix

gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Recommended articles