gb Reverse Transcription Kit

Reverse Transcription Kit is intended for reverse transcription using enzyme MMLV reverse transcriptase. This enzyme converts RNA to cDNA (complementary DNA) in one step (without denaturation), you only have to add your template RNA.

Description of the product

gb Reverse transcription kit contains buffer, dNTPs, primers (hexamers, oligo(dT)VN), RNAse inhibitor and enzyme reverse transcriptase (MMLV), that converts RNA to cDNA, which can be analyzed in the PCR. MMLV reverse transcriptase originates from Moloney Murine Leukemia Virus.

Parameters of the product

  • The product is prepared in a two-component form, it is enough to add only a sample of RNA after mixing the A component (solution) and B component (enzyme).
  • 2× concentrated mixture sufficient for 20 reactions is made by mixing both the components. RNA to cDNA transcription occurs in one step without the necessity of previous denaturation.
  • The input amount of RNA for reverse transcription is 100–1000 ng.
  • Reverse transcriptase is an M-MLV transcriptase which synthesizes cDNA molecules effectively up to 13 kilobases length at an optimum temperature of 42 °C.
  • There are poly(dT) oligonucleotides and degenerate hexamers as well in the mixture, which means the kit could be also used for reverse transcription of RNA without poly (A) tails.
Cat. No. Product No. of reactions Price
3012 gb Reverse Transcription Kit 20
Add to Quote

1 tube contains reagents to provide 20 reverse transcriptions (20 μl volume of each reaction).

Useful links
Recommended products
New
gb HRM PCR Master Mix

gb HRM PCR Master Mix gb HRM PCR Master Mix

gb HRM PCR Master Mix is intended for amplification of DNA with subsequent High Resolution Melting (HRM) analysis. HRM analysis is an efficient melt curve analysis method for genotyping, mutation scanning prior to sequencing analysis, DNA mapping, species identification or DNA methylation analysis etc.

More
gb Easy PCR Master Mix

gb Easy PCR Master Mix gb Easy PCR Master Mix

gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

More