Kit for isolation of DNA from microorganisms

The kit enables isolation of microbial DNA from pellet of bacterial cells, yeasts and fungi. It is possible to use it for genomic DNA isolation from different types of tissues of both human and animal origin. High DNA yields, reproducible results and simple working method are guaranteed.

Principle of the isolation kit

  • Cell lysis is reached by mechanical disruption using beating beads and followed by incubation of the particular sample with Proteinase K and solutions with a higher content of chaotropic ions.
  • DNA isolation is performed on the silica membrane of the column.
  • Specific conditions appropriate for binding DNA onto a membrane are achieved by ethanol added to lysate.
  • Consequent treatment by two types of buffer results in removing impurities in the first step and eluting pure genomic DNA in the next step (ratio A260/A280 1.70 to 1.90).

Description Specification
Input material cell pellet
Technology silica membrane column
Sample volume 102–108 cells
Elution volume 100 μl
Yield 20-35 μg
Binding capacity 60 μg
Size of DNA fragment 200 bp-30 kbp
Isolation time of 1 sample approx 60 min

 

Cat. No. Product No. of reactions Price
3103 Kit for isolation of DNA from microorganisms 50
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1 package contains materials and reagents for a total amount of 50 isolations.

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