Kit for isolation of DNA from tissues

The kit enables you to achieve highly pure genomic DNA from various types of human or animal tissues and cultured cells, buccal swabs or paraffin-embedded tissue. Moreover, microbial, fungal or yeast DNA can be efficiently isolated as well. High DNA yields, reproducible results and fast working method are guaranteed.

Principle of the isolation kit

  • Lysis of cells is caused by incubation of a sample in Proteinase K in the presence of increased content of chaotropic ions.
  • DNA isolation is performed on the silica membrane of the column.
  • Specific conditions appropriate for binding DNA onto a membrane are achieved by ethanol added to lysate.
  • Consequent treatment by two types of buffer results in removing impurities in the first step and eluting pure genomic DNA in the next step (ratio A260/A280 1.70 to 1.90).

Description Specification
Input material tissue, cultured cells, buccal swabs, paraffin-embedded tissue
Technology silica membrane column
Sample volume 1–25 mg tissue / cultured cells (102–107)
Elution volume 100 μl
Yield 20-35 μg
Binding capacity 60 μg
Size of DNA fragment 200 bp-30 kbp

 

Cat. No. Product No. of reactions Price
3101-050 Kit for isolation of DNA from tissues 50
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3101-100 Kit for isolation of DNA from tissues 100
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1 package contains material and chemicals for a total number of 50/100 DNA isolations from tissues. The kit can be used also for DNA isolation from microorganisms after adding beating beads

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