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qPCR assays for mouse reference genes

qPCR assays for mouse reference genes

The product is a lyophilized mixture of 2 primers and a hydrolysis probe. In combination with the reaction mixture (gb Master Mix according to your own choice or another reaction mixture of the customer) it is used for qPCR. By default, it is possible to order sets for human, rat and mouse housekeeping genes. Assays can be delivered separately for either individual genes or in the form of kits containing assays for 10 genes.

Principle of the assay

qPCR assay for reference genes are optimized systems for quantification of gene expression at the transcriptional level using real-time PCR. The goal is to measure the expression of the selected housekeeping gene that the customer uses to normalize expression of the target gene at relative quantification. The systems are designed and optimized in the same temperature PCR profile.

Product parameters

  • Lyophilized primers and probes (FAM and HEX labeling).
  • After reconstitution according to the instructions 10x concentrated solution.
  • Does not amplify genomic DNA (in case of pseudogenes occurrence the table contains a notice).
  • All assays have the same temperature profile.
  • qPCR standards
Cat. No. Product No. of reactions Price
3021-F Real-time PCR kit for 10 Mouse Reference Genes 10x100
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3021-H Real-time PCR kit for 10 Mouse Reference Genes 10x100
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* Package contains an amount sufficient for 100 / 10 x 100 qPCR reactions with a volume of 20 μl.

mouse qPCR assay - FAM

Cat. No. Product No. of reactions Price
3039-F mouse B2m (12010ˇ) 100
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3059-F mouse Gapdh* (14433ˇ) 100
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3040-F mouse Gusb (110006ˇ) 100
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3041-F mouse Hmbs (15288ˇ) 100
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3042-F mouse Hprt1* (15425ˇ) 100
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3043-F mouse Nup54* (269113ˇ) 100
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3044-F mouse Polr2a (20020ˇ) 100
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3045-F mouse Surf1 (20930ˇ) 100
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3046-F mouse Tbp (21374ˇ) 100
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3047-F mouse Ywhaz* (22631ˇ) 100
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* These kits can amplify genomic DNA because of the presence of pseudogenes.
ˇ Gene ID

mouse qPCR assay - HEX

Cat. No. Product No. of reactions Price
3039-H mouse B2m (12010ˇ) 100
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3059-H mouse Gapdh* (14433ˇ) 100
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3040-H mouse Gusb (110006ˇ) 100
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3041-H mouse Hmbs (15288ˇ) 100
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Add to Quote
3042-H mouse Hprt1* (15425ˇ) 100
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Add to Quote
3043-H mouse Nup54* (269113ˇ) 100
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Add to Quote
3044-H mouse Polr2a (20020ˇ) 100
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Add to Quote
3045-H mouse Surf1 (20930ˇ) 100
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Add to Quote
3046-H mouse Tbp (21374ˇ) 100
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Add to Quote
3047-H mouse Ywhaz* (22631ˇ) 100
Add to Quote
Add to Quote

* These kits can amplify genomic DNA because of the presence of pseudogenes.
ˇ Gene ID

Useful links
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