qPCR assays for rat reference genes

The product is a lyophilized mixture of 2 primers and a hydrolysis probe. In combination with the reaction mixture (gb Master Mix according to your own choice or another reaction mixture of the customer) it is used for qPCR. By default, it is possible to order sets for human, rat and mouse housekeeping genes. Assays can be delivered separately for either individual genes or in the form of kits containing assays for 10 genes.

Principle of the assay

qPCR assay for reference genes are optimized systems for quantification of gene expression at the transcriptional level using real-time PCR. The goal is to measure the expression of the selected housekeeping gene that the customer uses to normalize expression of the target gene at relative quantification. The systems are designed and optimized in the same temperature PCR profile.

Product parameters

  • Lyophilized primers and probes (FAM and HEX labeling).
  • After reconstitution according to the instructions 10x concentrated solution.
  • Does not amplify genomic DNA (in case of pseudogenes occurrence the table contains a notice).
  • All assays have the same temperature profile.
  • qPCR standards
Cat. No. Product No. of reactions Price
3022-F Real-time PCR kit for 10 Rat Reference Genes 10x100
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3022-H Real-time PCR kit for 10 Rat Reference Genes 10x100
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* Package contains an amount sufficient for 100 / 10 x 100 qPCR reactions with a volume of 20 μl.

rat qPCR assay - FAM

Cat. No. Product No. of reactions Price
3048-F rat B2m (24223ˇ) 100
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3061-F rat Gapdh* (24383ˇ) 100
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3049-F rat Gusb (24434ˇ) 100
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3050-F rat Hmbs (25709ˇ) 100
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3051-F rat Hprt1* (24465ˇ) 100
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3052-F rat Nup54* (53372ˇ) 100
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3053-F rat Polr2a (363633ˇ) 100
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3054-F rat Surf1 (64463ˇ) 100
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3055-F rat Tbp (117526ˇ) 100
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3056-F rat Ywhaz* (25578ˇ) 100
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* These kits can amplify genomic DNA because of the presence of pseudogenes.
ˇ Gene ID

rat qPCR assay - HEX

Cat. No. Product No. of reactions Price
3048-H rat B2m (24223ˇ) 100
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3061-H rat Gapdh* (24383ˇ) 100
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3049-H rat Gusb (24434ˇ) 100
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3050-H rat Hmbs (25709ˇ) 100
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3051-H rat Hprt1* (24465ˇ) 100
Add to Quote
3052-H rat Nup54* (53372ˇ) 100
Add to Quote
3053-H rat Polr2a (363633ˇ) 100
Add to Quote
3054-H rat Surf1 (64463ˇ) 100
Add to Quote
3055-H rat Tbp (117526ˇ) 100
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3056-H rat Ywhaz* (25578ˇ) 100
Add to Quote

* These kits can amplify genomic DNA because of the presence of pseudogenes.
ˇ Gene ID

Useful links
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