GENERI BIOTECH provides various types of oligonucleotide purification:
- Standard purification – final synthesis product is made free of chemical impurities only (excess of salts and protective groups). This type of purification is suitable for oligonucleotide lengths up to 40 – 50 bases.
- OPC purification (Oligonucleotide Purification Cartridge) – final synthesis product is made free of chemical impurities (excess of salts and protective groups) and also of shorter incomplete chains. This type of purification is sufficient for most standard applications.
- HPLC purification – oligonucleotide is very effectively separated from all impurities and improper-length sequences. HPLC purification provides highly pure oligonucleotide suitable even for the most demanding applications.
- HPLC plus purification – final synthesis product is made free of chemical impurities. This type of purification is sufficient for removing shorter unsynthesized chains including n-1 up to 10%.
- NGS purification – this is a combined removal of impurities suitable for the NGS method with a lower risk of cross-contamination.
Standard purification is not surcharged. Each oligo ordered with OPC or HPLC purification is surcharged with additional fee regardless delivery term, labelling or other modifications.
You can find out your prices of purified oligos using our oligo calculator (after you log-in to Customer Portal).
Guaranteed and usual yields of specified purification types (oligos 15-50 bases long)
SCALE 20 nmol | Guaranteed yield (nmol) | Usual yield (nmol) |
---|---|---|
standard purification | 10 | 13-20 |
OPC purification | 8 | 13-17 |
HPLC purification | N/A | N/A |
SCALE 40 nmol | Guaranteed yield (nmol) | Usual yield (nmol) |
---|---|---|
standard purification | 20 | 35-40 |
OPC purification | 10 | 30-35 |
HPLC purification | 5 | 10-35 |
SCALE 200 nmol | Guaranteed yield (nmol) | Usual yield (nmol) |
---|---|---|
standard purification | 60 | 130-180 |
OPC purification | 40 | 45-130 |
HPLC purification | 20 | 25-100 |
SCALE 1 µmol | Guaranteed yield (nmol) | Usual yield (nmol) |
---|---|---|
standard purification | 300 | 350-500 |
OPC purification | 100 | 200-300 |
HPLC purification | 50 | 100-250 |