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Purification of oligonucleotides

GENERI BIOTECH provides 3 types of oligonucleotide purification:

  • Standard purification – final synthesis product is made free of chemical impurities only (excess of salts and protective groups). This type of purification is suitable for oligonucleotide lengths up to 40 – 50 bases.
  • OPC purification (Oligonucleotide Purification Cartridge) – final synthesis product is made free of chemical impurities (excess of salts and protective groups) and also of shorter incomplete chains. This type of purification is sufficient for most standard applications.
  • HPLC purification – oligonucleotide is very effectively separated from all impurities and improper-length sequences. HPLC purification provides highly pure oligonucleotide suitable even for the most demanding applications.

Standard purification is not surcharged. Each oligo ordered with OPC or HPLC purification is surcharged with additional fee regardless delivery term, labelling or other modifications.

You can find out your prices of purified oligos using our oligo calculator (after you log-in to Customer Portal).

Guaranteed and usual yields of specified purification types (oligos 15-50 bases long)

SCALE 20 nmol Guaranteed yield (nmol) Usual yield (nmol)
standard purification 10 13-20
OPC purification 8 13-17
HPLC purification N/A N/A

 

SCALE 40 nmol Guaranteed yield (nmol) Usual yield (nmol)
standard purification 20 35-40
OPC purification 10 30-35
HPLC purification 5 10-35

 

SCALE 200 nmol Guaranteed yield (nmol) Usual yield (nmol)
standard purification 60 130-180
OPC purification 40 45-130
HPLC purification 20 25-100

 

SCALE 1 µmol Guaranteed yield (nmol) Usual yield (nmol)
standard purification 300 350-500
OPC purification 100 200-300
HPLC purification 50 100-250