Categories

Endotoxin detection

Endotoxin detection

This test reveals contamination by bacterial endotoxins (lipolysaccharide), which is a membrane component of gram-negative bacteria. This reaction is the basis of LAL test, used for detection and quantification of bacterial endotoxins. Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the Atlantic horseshoe crab, Limulus polyphemus.

It was later recognized that the animal’s blood cells contain granules with a clotting factor known as coagulogen; this is released outside the cell when bacterial endotoxin is encountered. Resulting coagulation is thought to contain bacterial infections in the animal’s semi-closed circulatory system.

LAL reacts with bacterial endotoxin lipopolysaccharide (LPS).

Test for endotoxins is performed in compliance with Europe pharmacopeia test 2.6.14.

Useful links
Recommended products
gb OneStep RT-qPCR Kit

gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit

gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More