gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
This test reveals contamination by bacterial endotoxins (lipolysaccharide), which is a membrane component of gram-negative bacteria. This reaction is the basis of LAL test, used for detection and quantification of bacterial endotoxins. Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the Atlantic horseshoe crab, Limulus polyphemus.
It was later recognized that the animal’s blood cells contain granules with a clotting factor known as coagulogen; this is released outside the cell when bacterial endotoxin is encountered. Resulting coagulation is thought to contain bacterial infections in the animal’s semi-closed circulatory system.
LAL reacts with bacterial endotoxin lipopolysaccharide (LPS).
Test for endotoxins is performed in compliance with Europe pharmacopeia test 2.6.14.