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OLIGO & PROBES

We are an EN ISO 9001, 13485 and 17025 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

The know-how we acquired in custom DNA oligo synthesis during the last 24 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.

Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.

Custom oligo synthesis

Custom oligo synthesis Custom oligo synthesis

In GENERI BIOTECH oligonucleotides are synthesized on Highly cross-linked polystyrene beads supports. Oligonucleotides thus synthesized have a very low representation of truncated shorter chains compared to normal CPG supports.

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GEMINI™ probes

GEMINI™ probes GEMINI™ probes

GEMINI™ probes are a type of TaqMan real-time PCR probe developed in GENERI BIOTECH. The technology of GEMINI is based on placing internal quenchers into the probe sequence which results in stronger quenching and lower background signal. The probes are HPLC purified (no additional fee).

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SEQplus sequencing primers

SEQplus sequencing primers SEQplus sequencing primers

5´-SEQplus modification of a sequencing primer enables reading of sequence from the 2nd nucleotide of the sequenced DNA. We offer this modification for any user´s sequence or as part of ready-to-use in-stock sequencing primers.

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Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

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Single-labeled hybridization probes – FRET probes

Single-labeled hybridization probes – FRET probes Single-labeled hybridization probes – FRET probes

The system of single-labeled probes (also called FRET probes - Fluorescence Resonance Energy Transfer) consists of a pair of probes, the former is labeled with donor fluorophore (3´FAM) at the 3 ́ end, the latter is labeled with acceptor fluorophore (5´CFR 610 or 635) at the 5 ́ end. The probes are designed to hybridize to the PCR target adjacent to each other. The probes are HPLC purified (no additional fee).

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NGS Primers

NGS Primers NGS Primers

Synthesis of primers for NGS (Next Generation Sequencing) including HPLC purification is available at advantageous prices.

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oligo DUPLEX

oligo DUPLEX oligo DUPLEX

We can prepare double-stranded DNA (oligo DUPLEX) from complementary single-stranded oligonucleotides (ssDNA). Duplex can be prepared from two oligonucleotides ordered by you, or we can design the complementary strand to the oligo sequence supplied by you.

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Inosin

Inosin Inosin

2'deoxyinosin is a "universal" base (it has the ability to bind to each of the 4 standard bases). It can be located anywhere in the oligonucleotide chain.

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2’F-RNA

2’F-RNA 2’F-RNA

2'-F-RNA is even more stable than 2'-O-methyl-RNA. 2'-F-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than RNA & 2'-O-methyl-RNA duplexes. It serves mainly for antisense applications. May be attached anywhere within the oligonucleotide sequence.

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2’OMe-RNA

2’OMe-RNA 2’OMe-RNA

Compared to RNA 2'-O-methyl-RNA is more chemically stable (being stable against nucleases for several days). 2'-O-methyl-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than DNA & RNA duplexes. It is used mainly in antisense applications. May be attached anywhere within the oligonucleotide sequence.

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2’3′-ddC

2’3′-ddC 2’3′-ddC

It serves as a blocker of 3' polymerase extension. 2',3'-ddC may be attached to the 3' end of the oligonucleotide.

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