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LIFE SCIENCES

Our product line Life Sciences comprises  oligonucleotides in many categories, the most commonly used products for molecular biology (such as DNA isolation kits, PCR mastermixes, reverse transcription kits etc.) including products specialized for real-time PCR (assays for reference genes, assays on demand etc.).

Our Life Sciences line comprises some services for R&D as well.

Our typical partners are end-users of the products (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy (see here).

gb HRM PCR Master Mix

gb HRM PCR Master Mix gb HRM PCR Master Mix

gb HRM PCR Master Mix falls into a group of Dye-Based Real-time PCR Master Mixes. The product is intended for amplification of DNA with subsequent High Resolution Melting (HRM) analysis. HRM analysis is an efficient melt curve analysis method for genotyping, mutation scanning prior to sequencing analysis, DNA mapping, species identification or DNA methylation analysis etc.

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gb IPC PCR Master Mix

gb IPC PCR Master Mix gb IPC PCR Master Mix

gb IPC PCR Master Mix falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and additives that prevent PCR inhibition and Internal positive control (IPC). IPC included in the Master Mix serves to verify that the PCR procedure was done properly.

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gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

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gb Elite Reverse Transcription Kit

gb Elite Reverse Transcription Kit gb Elite Reverse Transcription Kit

gb Elite Reverse Transcription Kit is intended for reverse transcription using enzyme MMLV reverse transcriptase. This enzyme converts RNA to cDNA (complementary DNA) in one step (without denaturation), you only have to add your template RNA.

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Authentication of cell lines

Authentication of cell lines Authentication of cell lines

Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.

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gb Ideal PCR Master Mix

gb Ideal PCR Master Mix gb Ideal PCR Master Mix

gb Ideal PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.

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Plasmid amplification and isolation

Plasmid amplification and isolation Plasmid amplification and isolation

Plasmid amplification is provided in Escherichia coli bacteria cells. Plasmid linearization by restriction cleavage can be ordered as a follow-up service. Such an operation is recommended especially when the plasmid is used as a PCR standard. Amplified plasmids are delivered either in midiprep or maxiprep quantities. Typical yields are stated below.

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Kit for isolation of DNA from body fluids

Kit for isolation of DNA from body fluids Kit for isolation of DNA from body fluids

The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed.

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Kit for isolation of DNA from tissues

Kit for isolation of DNA from tissues Kit for isolation of DNA from tissues

The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from various types of human or animal tissues and cultured cells, buccal swabs or paraffin-embedded tissue. Moreover, microbial, fungal or yeast DNA can be efficiently isolated as well. High DNA yields, reproducible results and fast working method are guaranteed.

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Kit for isolation of DNA from microorganisms

Kit for isolation of DNA from microorganisms Kit for isolation of DNA from microorganisms

The DNA extraction kit includes DNA purification step and enables isolation of microbial DNA from pellet of bacterial cells, yeasts and fungi. It is possible to use it for genomic DNA isolation from different types of tissues of both human and animal origin. High DNA yields, reproducible results and simple working method are guaranteed.

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Beating beads

Beating beads Beating beads

Intended for mechanical disruption of the cell walls of microorganisms when DNA extraction is performed.

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Plasmid linearization

Plasmid linearization Plasmid linearization

If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.

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