gb Basic PCR Master Mix

gb Basic PCR Master Mix

gb Basic PCR Master Mix is intended for research end-point PCR applications. Beneficial feature is its enhanced resistance to PCR inhibitors. It is not intended for use in diagnostics.

Description of the product

gb Basic PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but is quickly activated during the initial denaturation step of PCR.

Parameters of the product

  • Master Mix is a 2× concentrated solution.
  • It contains all the components necessary for PCR performance. It is specified especially for real-time PCR applications demanding high sensitivity, for example microbial DNA detection and somatic mutations detection.
  • Polymerase is a hot-start type with a short activation time (3 min / 95 °C), with 5´-3´ polymerase and exonuclease activity, 3´-5´ exonuclease activity is not present.


gb PCR Master Mix by application
gb Basic
end-point PCR, common PCR amplification

real-time PCR without probes
real-time PCR with hydrolysis probes
real-time PCR with LNA probes
real-time PCR with hybridization probes

real-time PCR with High Resolution Melting Analysis
real-time PCR with low DNA samples
real-time PCR with inhibited samples

Cat. No. Product No. of reactions Price
3004 gb Basic PCR Master Mix 100
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

General Terms and Conditions

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Real-Time PCR Troubleshooting

Real-Time PCR Troubleshooting

PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.

gb HRM PCR Master Mix

gb HRM PCR Master Mix gb HRM PCR Master Mix

gb HRM PCR Master Mix falls into a group of Dye-Based Real-time PCR Master Mixes. The product is intended for amplification of DNA with subsequent High Resolution Melting (HRM) analysis. HRM analysis is an efficient melt curve analysis method for genotyping, mutation scanning prior to sequencing analysis, DNA mapping, species identification or DNA methylation analysis etc.