Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of mutation G1691A (Leiden) in coagulation factor V in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Coagulation factor V is a plasmatic glycoprotein involved in the process of blood coagulation. In its activated form, factor V participates in the conversion of prothrombin to thrombin which contributes to the creation of blood clots. Mutation G1691A (so-called Leiden mutation) causes replacement of amino acid arginine by glutamine in the position 506 of the factor V protein chain.
The frequency of the Leiden mutation in the healthy European population ranges between 3–5 %, whereas in patients who suffer from thromboembolic events (TEN) this frequency is about 20–40 %. The risk of venous thrombosis increases 3–10× in patients with heterozygote genotype and 20–80× in mutated homozygotes.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3200-025 | gb HEMO FV (G1691A) | 25 |
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| 3200 | gb HEMO FV (G1691A) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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Validated for:
ABI 7500/7500 Fast (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
Stratagene Mx3000P/Mx30005P (Agilent Technologies)
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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