Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of C807T mutation in GPIa gene in human genomic DNA. Detection is based on realtime polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Platelet glycoprotein GPIa forms a complex GPIa/IIa with glycoprotein GPIIa on the surface of blood platelets. One-nucleotide C807T mutation is one of the “silent” polymorphisms that do not change the amino acid sequence. However, the presence of the mutated form increases the gene expression.
Both, heterozygous and homozygous carriers of the mutated allele, have twice as many receptors on the platelet surface as compared to the wild type homozygotes, which causes an increased thrombocyte adhesivity to type 1 collagen. Under high shear stress in the arterial bed, increased thrombocyte adhesivity has thrombogenic potential. Both, heterozygous and homozygous carriage of the mutated allele, is considered to be a mild risk factor of myocardial infarction and stroke in younger people.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3211-025 | gb HEMO GPIa (C807T) | 25 |
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| 3211 | gb HEMO GPIa (C807T) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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Validated for:
ABI 7300 (ABI)
ABI 7500/7500 Fast (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
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The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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