Detekční metoda: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of mutation C677T in MTHFR gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Methylenetetrahydrofolate reductase (MTHFR) is the main regulatory enzyme involved in homocysteine and folic acid (folates) metabolisms. This mutation leads to enzyme activity reduction, increase of homocysteine level in blood and methionine deficiency. However, lower catalytic activity of MTHFR enzyme means higher utilisation of 5, 10-methylenetetrahydrofolate in DNA synthesis or repair.
The risk of venous thrombosis increases in combination with other risk factors (e.g. mutation in MTHFR gene – A1298C, mutation G20210A in factor II, Leiden mutation, long-time immobility, incorrect regimen, hormonal contraception) and grows with the number of mutated alleles in genotype.
| Kat. číslo | Produkt | No. of reactions | Cena |
|---|
| 3202-025 | gb HEMO MTHFR (C677T) | 25 |
Do poptávky Do poptávky |
| 3202 | gb HEMO MTHFR (C677T) | 100 |
Do poptávky Do poptávky |
1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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€ 115 (EU)
For other destinations see
indicative list.
Všeobecné obchodní podmínky
Validováno pro:
ABI 7500/7500 Fast (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
Stratagene Mx3000P/Mx30005P (Agilent Technologies)
Kit for isolation of DNA from body fluids The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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