Available synthesis scales for custom DNA oligos
20 nmol, 40 nmol, 200 nmol, 1 μmol and 10 μmol
Chain length
The standard length range of synthesized oligo is 2-99 bases.
For unmodified oligonucleotides in 20 nmol scale, synthesis is available up to 50 bases in length.
Delivery time for custom oligonucleotide synthesis
A – preferred synthesis
B – common synthesis more about delivery time
Degeneration
Oligonucleotides may contain degeneration in any position. Degeneration can be performed as a mixture of bases or insertion of inosine into the chain. Insertion of inosine into a string: see the modification Inosin.
Marking a degenerate position in the order:
I = inosin |
R = A, G |
N = A,C,G,T |
Y = C,T |
V = G,A,C |
M = A,C |
D = G,A,T |
K = G,T |
H = A,T,C |
S = G,C |
B = G,T,C |
W = A,T |
The final product of custom oligo synthesis service
The quantity of oligonucleotides is determined by UV spectrophotometry and expressed in nanomoles, optical units and micrograms. We standardly supply oligonucleotides in the dried state. Some oligonucleotides are delivered in a non-dried state for technological reasons.
A regular part of the production process is standard purification (desalination, removal of chemical impurities). The oligonucleotides of common lengths (up to 40 bases) are of such quality that no other purification is necessary for conventional applications in molecular biology.