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Kit for isolation of DNA from body fluids

The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed. Sample preparation buffers included.

Principle of the isolation kit

Lysis of cells is caused by incubation of a sample in Proteinase K in the presence of increased content of chaotropic ions.
DNA isolation is performed on the silica membrane of the column.
Specific conditions appropriate for binding DNA onto a membrane are achieved by ethanol added to lysate.
Consequent treatment by two types of buffer results in removing impurities in the first step and eluting pure genomic DNA in the next step (ratio A260/A280 1.60 to 1.90).

Description	Specification
Input material	whole blood (in EDTA, citrate or heparin), serum, plasma, buccal swab, urine
Technology	silica membrane column
Sample volume	up to 200 μl
Elution volume	100 μl
Yield	4-6 μg
Binding capacity	60 μg
Size of DNA fragment	200 bp-50 kbp
Isolation time of 1 sample	approx 20 min

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Cat. No.	Product	No. of isolations	Price

3100-050

Kit for isolation of DNA from body fluids

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3100-100

Kit for isolation of DNA from body fluids

100

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1 package contains material and chemicals for a total number of 50/100 DNA extraction (including DNA purification) from blood and other body fluids.

Shiping at ambient temperature from € 40 (EU)
For other destinations see indicative list.

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General Terms and Conditions

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