gb OneStep IPC Elite RT-qPCR Kit contains hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and M-MuLV reverse transcriptase and internal positive control (IPC). The IPC contained in the Master Mix is used to check the correctness of the PCR, allowing the user to distinguish a sample that appears to be negative due to poorly passed PCR, from a truly negative sample.
gb OneStep IPC Elite RT-qPCR Kit is intended for high sensitivity reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e.onestep RT-qPCR. No additional instrumentation is required; the onestep RT-qPCR analysis can be performed directly on any real-time PCR cycler simply by adding a thermal step for reverse transcription prior to a PCR amplification.
The IPC signal is detected in the HEX / JOE / VIC fluorescence channel. gb OneStep IPC RT-qPCR Kit does not contain any passive reference dye, it can be added to the reaction mixture separately (view Passive Reference Dye, Cat. No. 3010 – on request).
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3019-050 | gb OneStep IPC Elite RT-qPCR Kit | 50 |
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| 3019-500 | gb OneStep IPC Elite RT-qPCR Kit | 500 |
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1 pack contains reagents to provide 50/500 reverse transcriptions (20 μl volume of each reaction).
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€ 110 (EU)
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indicative list.
General Terms and Conditions
PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.
gb Elite Reverse Transcription Kit gb Elite Reverse Transcription Kit is intended for reverse transcription using enzyme MMLV reverse transcriptase. This enzyme converts RNA to cDNA (complementary DNA) in one step (without denaturation), you only have to add your template RNA.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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