gb GelReady PCR Master Mix is intended for end-point PCR applications with product visualization by gel electrophoresis. Master Mix contains dyes and additives for direct loading of the PCR product into the gel without adding a loading buffer.
gb GelReady PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTPs, MgCl2, dyes and additives for direct loading of the PCR product into the gel. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at the room temperature but it is rapidly activated during the initial denaturation step of PCR. gb GelReady PCR Master Mix is not suitable for use in real-time PCR applications. This product is intended for research use only.
| gb PCR Master Mix by application |
gb GelReady |
| end-point PCR, common PCR amplification |
✓ |
| real-time PCR without probes | |
| real-time PCR with hydrolysis probes | |
| real-time PCR with LNA probes | |
| real-time PCR with hybridization probes | |
| real-time PCR with High Resolution Melting Analysis | |
| real-time PCR with low DNA samples | |
| real-time PCR with inhibited samples |
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3009 | gb GelReady PCR Master Mix | 100 |
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).
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€ 110 (EU)
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indicative list.
General Terms and Conditions
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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