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gb GelReady PCR Master Mix

gb GelReady PCR Master Mix

gb GelReady PCR Master Mix is ​​intended for end-point PCR applications with product visualization by gel electrophoresis. Master Mix contains dyes and additives for direct loading of the PCR product into the gel without adding a loading buffer.

Description of the product

gb GelReady PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTPs, MgCl2, dyes and additives for direct loading of the PCR product into the gel. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at the room temperature but it is rapidly activated during the initial denaturation step of PCR. gb GelReady PCR Master Mix is not suitable for use in real-time PCR applications. This product is intended for research use only.

Parametres of the product

  • PCR Master Mix is 2× concentrated
  • One vial contains 1 ml of PCR Master Mix sufficient for 100 of PCR reactions of 20 µl
  • PCR Master Mix contains the Taq DNA polymerase, PCR reaction buffer, dNTPs, MgCl2, dyes and additives
  • Taq DNA polymerase is a hot-start type with a short activation time, with the 5´-3´ polymerase and exonuclease activity, lacking the 3´-5´ exonuclease activity.
  • PCR Mater Mix is suitable for end-point PCR analysis with a subsequent product visualization by gel electrophoresis
gb PCR Master Mix by application
gb GelReady
end-point PCR, common PCR amplification

real-time PCR without probes

real-time PCR with hydrolysis probes

real-time PCR with LNA probes

real-time PCR with hybridization probes

real-time PCR with High Resolution Melting Analysis
real-time PCR with low DNA samples
real-time PCR with inhibited samples

Cat. No. Product No. of reactions Price
3009 gb GelReady PCR Master Mix 100
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 100 (EU)
For other destinations see indicative list.

Useful links
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gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.

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