Mycoplasma detection – biochemical test
The test is intended to detect mycoplasma in cell culture media.
Our product line Life Sciences comprises the most commonly used products for molecular biology (such as DNA isolation kits, PCR mastermixes, one-step reverse transcription kits etc.) including products specialized for real-time PCR. Our Life Sciences line comprises some services for R&D as well.
Our typical partners are end-users of the products (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy (see here).
The test is intended to detect mycoplasma in cell culture media.
If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.
This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.
The service is complementary for plasmids prepared in GENERI BIOTECH. It includes storage of a bacterial culture containing the plasmid. The standard storage period is one year.
Passive reference dye is an inert dye which serves as inner reference dye for fluorescence signal normalization between the wells which can occur as a result of errors in pipetting or under the influence of device limitations. It is used with TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR or with probe-based One-Step qRT-PCR.
gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
gb PCR Tips with Filter are sterile, DNase free, RNase free, pyrogen free. Tips are packed in a 96-piece rack. They are compatible with commonly used pipettes of various brands. The smallest available size is 1 pack containing 10 racks (960 tips).
gb Basic Reverse Transcription Kit is intended for reverse transcription using enzyme MMLV reverse transcriptase. This enzyme converts RNA to cDNA (complementary DNA) in one step (without denaturation), you only have to add your template RNA.
gb OneStep IPC Elite RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis and qPCR in one-step. The product is intended for high sensitivity reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required. The kit contains IPC.
gb GelReady PCR Master Mix is intended for end-point PCR applications with product visualization by gel electrophoresis. Master Mix contains dyes and additives for direct loading of the PCR product into the gel without adding a loading buffer.
The method involves isolating DNA from a sample of a conditioned eukaryotic cell medium. Subsequently, amplification and detection of a highly conserved region encoding 16S ribosomal RNA specific for mycoplasmas is performed by real-time PCR. This method is used for the qualitative determination of mycoplasmas in a sample.
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