This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.
The following methods are most commonly used to verify accuracy of a plasmid:
1) Restriction analysis enabling the recognition of more extensive deletions (typically if a target sequence was not cloned in) or an accidental interchange of plasmids (typically if plasmids do not originate from an original source).
2) Plasmid conformation determination suitable for quality check of a native plasmid. When native, a plasmid should be present prevailingly in supercoiled conformation. In order to verify the conformation, plasmid analysis on gel is used and a percentage of the supercoiled form is determined.
Plasmid verification can also be performed under GMP or GLP regulations.
We provide the customer with a reporting protocol. If the customer determines acceptability criteria, it is possible to issue a report stating that the plasmid complies with the determined parameters.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1855-R | Plasmid verification by restriction analysis |
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| 1855-C | Plasmid verification by conformation determination |
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€ 40 (EU)
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General Terms and Conditions
Kit for isolation of DNA from body fluids The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Test output is in the form of a reporting protocol. If the customer provides so-called acceptability criteria, it is possible to issue a report stating that plasmid meets/does not meet these criteria.
Dispatch date
According to requirements on the method for plasmid verification
Shipping
Reporting protocol in a printed version – by Czech Post or electronically by email
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