Home Products Plasmid verification

Plasmid verification

This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.

Description of the service

The following methods are most commonly used to verify accuracy of a plasmid:

1) Restriction analysis enabling the recognition of more extensive deletions (typically if a target sequence was not cloned in) or an accidental interchange of plasmids (typically if plasmids do not originate from an original source).

2) Plasmid conformation determination suitable for quality check of a native plasmid. When native, a plasmid should be present prevailingly in supercoiled conformation. In order to verify the conformation, plasmid analysis on gel is used and a percentage of the supercoiled form is determined.

Plasmid verification can also be performed under GMP or GLP regulations.

Output of the test

We provide the customer with a reporting protocol. If the customer determines acceptability criteria, it is possible to issue a report stating that the plasmid complies with the determined parameters.

View allView less

Cat. No.	Product	No. of reactions	Price

1855-R

Plasmid verification by restriction analysis

Add to Quote
Add to Quote

1855-C

Plasmid verification by conformation determination

Add to Quote
Add to Quote

Shiping at ambient temperature from € 40 (EU)
For other destinations see indicative list.

Download Product Information Download Full Catalogue

General Terms and Conditions

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.