The system of single-labeled probes (also called FRET probes – Fluorescence Resonance Energy Transfer) consists of a pair of probes, the former is labeled with donor fluorophore (3´FAM) at the 3 ́ end, the latter is labeled with acceptor fluorophore (5´CFR 610 or 635) at the 5 ́ end. The probes are designed to hybridize PCR targets adjacent to each other. The probes are HPLC purified (no additional fee).
Available amounts: 1 nmol, 2 nmol and 5 nmol
| Cat. No. | Product | Amount | Price |
|---|
| 1508-001 | FAM | 1 nmol |
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| 1508-002 | FAM | 2 nmol |
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| 1508-005 | FAM | 5 nmol |
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probe structure: 3′ FAM
The probe serves in pair with CFR 610 or CFR 635.
| Cat. No. | Product | Amount | Price |
|---|
| 1515-001 | CFR 610 | 1 nmol |
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| 1515-002 | CFR 610 | 2 nmol |
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| 1515-005 | CFR 610 | 5 nmol |
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probe structure: 3′ phosphate – 5′ CFR 610
CFR 610: an alternative to LC red 610 and Texas Red
| Cat. No. | Product | Amount | Price |
|---|
| 1509-001 | CFR 635 | 1 nmol |
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| 1509-002 | CFR 635 | 2 nmol |
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| 1509-005 | CFR 635 | 5 nmol |
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probe structure: 3′ phosphate – 5′ CFR 635
CFR 635: an alternative to LC red 640
Shiping at ambient temperature from
€ 40 (EU)
For other destinations see
indicative list.
General Terms and Conditions
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Term A: quick term not available
Term B: products are dispatched within the next 5 full business days after receiving the order
Shipping
At room temperature
Storage recommendation
Dried oligonucleotides: at 4 °C (in a refrigerator)
Dissolved: -20°C (in a freezer), we recommend to store in aliquotes
In a dark place (without exposure to the light)
Product expiry date
24 months from the date of manufacture (provided the product is stored as recommended)
The provided information is only indicative and is always subject to the specific content and extent of an order.
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