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Single-labeled hybridization probes – FRET probes

Single-labeled hybridization probes – FRET probes

The system of single-labeled probes (also called FRET probes – Fluorescence Resonance Energy Transfer) consists of a pair of probes, the former is labeled with donor fluorophore (3´FAM) at the 3 ́ end, the latter is labeled with acceptor fluorophore (5´CFR 610 or 635) at the 5 ́ end. The probes are designed to hybridize PCR targets adjacent to each other. The probes are HPLC purified (no additional fee).

Available amounts: 1 nmol, 2 nmol and 5 nmol

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Cat. No. Product Amount Price
1508-001 FAM 1 nmol
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1508-002 FAM 2 nmol
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1508-005 FAM 5 nmol
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probe structure: 3′ FAM

The probe serves in pair with CFR 610 or CFR 635.

Probe CFR 610

Cat. No. Product Amount Price
1515-001 CFR 610 1 nmol
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1515-002 CFR 610 2 nmol
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1515-005 CFR 610 5 nmol
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probe structure: 3′ phosphate – 5′ CFR 610

CFR 610: an alternative to LC red 610 and Texas Red

Probe CFR 635

Cat. No. Product Amount Price
1509-001 CFR 635 1 nmol
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1509-002 CFR 635 2 nmol
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1509-005 CFR 635 5 nmol
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probe structure: 3′ phosphate – 5′ CFR 635

CFR 635: an alternative to LC red 640

Shiping at ambient temperature from € 40 (EU)
For other destinations see indicative list.

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Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

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